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KMID : 0380020030180010070
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Korean Journal of Biotechnology and Bioengineering
2003 Volume.18 No. 1 p.70 ~ p.73
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Simple and Quantitative Analysis Method for Lactic Add by TLC
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Choi Mi-Hwa
Cho Gap-Soo
Kang Hee-Kyung
Yun Jong-Sun
Seo Eun-Sung
Ryu Hwa-Won
Chang Se-Hyo
Yun Seong-Hun
Kim Do-Man
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Abstract
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TLC condition was developed for its simple separation and quantitative analysis of lactic acid. Rapid and clear separation of lactic acid by silica gel TLC plate was obtained by using nitromethane : 1-propanol : (2 : 5 : 1.5, v/v/v) and a suitable dipping solution of 40 mg bromocresol purple in 100 mL 5% ethanol (pH 10.0). The lactic acid was shown as a bright yellow spot on a light cinnabar background. The quantitatively detectable concentration range of lactic acid was between 0.5 and 4% with 99.4%, confidence. Quantitative TLC analysis result was confirmed with HPLC and with enzymatic Quantitative analysis methods (by using lactate dehydrogenase).
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KEYWORD
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TLC, Thin layer chromatography, lactic acid, bromocresol purple, quantitative analysis method, separation
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